Summary
Type: SPE
Application #: 305700
Catalog#: 730011 - SPE Cartridges, Chromabond, C18 ec, 1ml, PP, 100mg, 100/pk
Detection:
Conditions: Column conditioning: 1000 µL methanol, then 1000 µL 25 mmol/L phosphate buffer, pH 5.5; (Do not let run the column dry.)
Sample aspiration: 1000 µL of aqueous sample or approx. 700 µL of blood plasma sample was loaded into the column
Washing: 300 µL 25 mmol/L phosphate buffer, then 300 µL water, and for blood samples additionally with hexane
Drying: nitrogen, 40 °C, 15 min (SpeedVac)
Elution: 2 x 300 µL methanol
Evaporation: 40 °C, 60 min (SpeedVac)
Reconstitution: 100 µL mobile HPLC phase; shaken (2400 shakes/min, 2 min); centrifuged (14100 g, 1 min)
Authors: Bergman, N.
Source: Bachelor´s Thesis, Linköpings University, Sweden